Monitoring autophagic flux in Caenorhabditis elegans using a p62/SQST-1 reporter

Christina Ploumi; Aggeliki Sotiriou; Nektarios Tavernarakis

doi:10.1016/bs.mcb.2020.10.011

Monitoring autophagic flux in Caenorhabditis elegans using a p62/SQST-1 reporter

Methods Cell Biol. 2021:165:73-87. doi: 10.1016/bs.mcb.2020.10.011. Epub 2020 Nov 18.

Authors

Christina Ploumi¹, Aggeliki Sotiriou¹, Nektarios Tavernarakis²

Affiliations

¹ Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Crete, Greece; Department of Basic Sciences, Faculty of Medicine, University of Crete, Heraklion, Crete, Greece.
² Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Crete, Greece; Department of Basic Sciences, Faculty of Medicine, University of Crete, Heraklion, Crete, Greece. Electronic address: tavernarakis@imbb.forth.gr.

PMID: 34311872
DOI: 10.1016/bs.mcb.2020.10.011

Abstract

Autophagy is a well-conserved self-degrading mechanism, which involves the elimination of unnecessary or damaged cellular constituents. Although extensively studied, many aspects regarding its tight regulation and its implication in health and disease remain elusive. The nematode Caenorhabditis elegans has been widely used as a simple multicellular model organism for studying the autophagic machinery per se, and uncover its multidimensional roles in the maintenance of cellular and organismal homeostasis. The current protocol describes the in vivo detection and biochemical analysis of the autophagic substrate SQST-1, as an indicator of autophagic flux in C. elegans.

Keywords: Autophagic flux; Autophagy; Caenorhabditis elegans; LC3; SQST-1; Sequestosome; p62.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Autophagy
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans* / genetics

Substances

Caenorhabditis elegans Proteins