Analysis of amino acids in cell culture supernatant using capillary electrophoresis with contactless conductivity detection

Saara Mikkonen; Åsa Emmer

doi:10.1002/elps.202100122

Analysis of amino acids in cell culture supernatant using capillary electrophoresis with contactless conductivity detection

Electrophoresis. 2021 Oct;42(19):1924-1927. doi: 10.1002/elps.202100122. Epub 2021 Aug 6.

Authors

Saara Mikkonen¹, Åsa Emmer¹

Affiliation

¹ Division of Applied Physical Chemistry, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden.

PMID: 34311502
DOI: 10.1002/elps.202100122

Abstract

CE-C⁴ D methods for the analysis of amino acids (AAs) are presented. Combining the results from two methods with acetic acid and cyclodextrin-based BGEs, 20 proteinogenic AAs could be analyzed using CE. CE-C⁴ D was also, for the first time, applied to analyze free AAs in samples of mammalian cell culture supernatant. After dilution as only sample preparation, combining the results of the two CE methods allowed monitoring the concentration changes of 17 AAs in samples taken during the cultivation of CHO cells.

Keywords: Amino acid; Capillary electrophoresis; Cell culture; Contactless conductivity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids*
Animals
Cell Culture Techniques
Cricetinae
Cricetulus
Electric Conductivity
Electrophoresis, Capillary*

Substances

Amino Acids