Dyadobacter subterraneus sp. nov., isolated from hydrocarbon-polluted groundwater from an oil refinery in Hungary

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, rod-shaped bacterial strain (UP-52^T) was isolated from hydrocarbon-polluted groundwater located near an oil refinery in Tiszaujvaros, Hungary. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Dyadobacter in the family Cytophagaceae. Its closely related species are Dyadobacter frigoris (98.00 %), Dyadobacter koreensis (97.64 %), Dyadobacter psychrophilus (97.57 %), Dyadobacter ginsengisoli (97.56 %) and Dyadobacter psychrotolerans (97.20 %). The predominant fatty acids are summed feature 3 (C_{16 : 1} ω7c and/or C_{16 : 1} ω7c/C_{16 : 1} ω6c), C_{15 : 0} iso, C_{16 : 1} ω5c and C_{17 : 0} iso 3OH. The predominant respiratory quinone detected in strain UP-52^T is quinone MK-7. The dominant polar lipids are glycolipid, phosphoaminolipid, phospholipid and aminolipid. The DNA G+C content is 40.0 mol%. Flexirubin-type pigment was present. Based on these phenotypic, chemotaxonomic and phylogenetic results, UP-52^T represents a novel species of the genus Dyadobacter, for which the name Dyadobacter subterraneus sp. nov. is proposed. The type strain is UP-52^T (=NCAIM B.02653^T=CCM 9030^T).

Keywords: Dyadobacter; Hungary; Tiszaujvaros; hydrocabon-contaminated groundwater; multidrug-resistant.