A protocol for characterization of extremely preterm infant gut microbiota in double-blind clinical trials

STAR Protoc. 2021 Jul 9;2(3):100652. doi: 10.1016/j.xpro.2021.100652. eCollection 2021 Sep 17.

Abstract

16S rRNA gene sequencing enables microbial community profiling, but recovering fecal DNA from extremely premature infants is challenging. Here, we describe an optimized protocol for fecal DNA isolation, library preparation for 16S rRNA gene sequencing, taxonomy assignation, and statistical analyses. The protocol is complemented with a quantitative PCR for probiotic L. reuteri identification. This protocol describes how to characterize preterm infant gut microbiota and relate it to probiotic supplementation and clinical outcomes. It is customizable for other clinical trials. For complete details on the use and execution of this protocol, please refer to Martí et al. (2021) and Spreckels et al. (2021).

Keywords: Clinical Protocol; Health Sciences; Microbiology; Molecular Biology; Sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Clinical Trials as Topic
  • DNA / isolation & purification
  • Double-Blind Method
  • Feces / microbiology*
  • Gastrointestinal Microbiome / genetics
  • Gastrointestinal Microbiome / physiology*
  • Humans
  • Infant, Extremely Premature / physiology*
  • Infant, Newborn
  • Limosilactobacillus reuteri / genetics
  • Polymerase Chain Reaction / methods*
  • Probiotics
  • RNA, Ribosomal, 16S / genetics*

Substances

  • RNA, Ribosomal, 16S
  • DNA