Alteration of N6-Methyladenosine RNA Profiles in Cisplatin-Induced Acute Kidney Injury in Mice

Can-Ming Li; Ming Li; Wen-Bo Zhao; Zeng-Chun Ye; Hui Peng

doi:10.3389/fmolb.2021.654465

Alteration of N6-Methyladenosine RNA Profiles in Cisplatin-Induced Acute Kidney Injury in Mice

Front Mol Biosci. 2021 Jul 9:8:654465. doi: 10.3389/fmolb.2021.654465. eCollection 2021.

Authors

Can-Ming Li¹, Ming Li¹, Wen-Bo Zhao¹, Zeng-Chun Ye¹, Hui Peng¹

Affiliation

¹ Department of Nephrology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Abstract

Aim: To identify the alterations of N6-methyladenosine (m⁶A) RNA profiles in cisplatin-induced acute kidney injury (Cis-AKI) in mice. Materials and Methods: The total level of m⁶A and the expression of methyltransferases and demethylases in the kidneys were measured. The profiles of methylated RNAs were determined by the microarray method. Bioinformatics analysis was performed to predict the functions. Results: Global m⁶A levels were increased after cisplatin treatment, accompanied by the alterations of Mettl3, Mettl14, Wtap, Fto, and Alkbh5. A total of 618 mRNAs and 98 lncRNAs were significantly differentially methylated in response to cisplatin treatment. Bioinformatics analysis indicated that the methylated mRNAs predominantly acted on the metabolic process. Conclusion: M⁶A epitranscriptome might be significantly altered in Cis-AKI, which is potentially implicated in the development of nephrotoxicity.

Keywords: N6-methyladenosine (m6A); RNA; acute kidney injury; cisplatin; microarray.