CHOP overexpression sensitizes human non-small cell lung cancer cells to cisplatin treatment by Bcl-2/JNK pathway

Li-Le Wang; Rui-Cheng Hu; Ai-Guo Dai; Shuang-Xiang Tan; Ming Xu; Chun-Chu Kong; Yun-Rong Chen; Dai-Yan Fu

CHOP overexpression sensitizes human non-small cell lung cancer cells to cisplatin treatment by Bcl-2/JNK pathway

Am J Transl Res. 2021 Jun 15;13(6):6279-6287. eCollection 2021.

Authors

Li-Le Wang¹, Rui-Cheng Hu¹, Ai-Guo Dai², Shuang-Xiang Tan¹, Ming Xu³, Chun-Chu Kong¹, Yun-Rong Chen¹, Dai-Yan Fu¹

Affiliations

¹ Department of Respiratory Medicine, Hunan Provincial People's Hospital/The First Affiliated Hospital of Hunan Normal University Changsha 410016, China.
² Department of Respiratory Diseases, Medical School, Hunan University of Chinese Medicine Changsha 410208, China.
³ Department of Gastroenterology Medicine, Hunan Provincial People's Hospital/The First Affiliated Hospital of Hunan Normal University Changsha 410016, China.

PMID: 34306367
PMCID: PMC8290817

Abstract

C/EBP homologous protein (CHOP), a 29 kDa cellular protein, plays a role in regulating tumor proliferation, differentiation, metabolism, cell death, and in tumor resistance to chemotherapy. Non-small cell lung cancer (NSCLC) is a tumor of the respiratory system and drug resistance is prevalent among NSCLC clinical cell cultures. Herein, our study elucidated the effect of CHOP on NSCLC cells with cisplatin resistance and its mechanism. In a NSCLC cell line with cisplatin-resistance, CHOP expression was decreased, compared with A549 cells. Overexpression of CHOP decreased the cell viability and enhanced cell apoptosis in the cells treated with cisplatin. Expression of CHOP also inhibited the cell proliferation and metastasis. CHOP increased the therapeutic effect of cisplatin on NSCLC cells through the Bcl-2/JNK pathway. In summary, CHOP regulated cisplatin resistance in cells of NSCLC by promoting the expression of apoptotic proteins and inhibiting the Bcl-2/JNK signaling pathway, indicating the antitumor effects of CHOP.

Keywords: Bcl-2; CHOP; JNK; NSCLC; apoptosis; cisplatin.