miR-374a-5p inhibits non-small cell lung cancer cell proliferation and migration via targeting NCK1

Qiang Guo; Haijun Wang; Yanzhao Xu; Mingbo Wang; Ziqiang Tian

doi:10.3892/etm.2021.10375

miR-374a-5p inhibits non-small cell lung cancer cell proliferation and migration via targeting NCK1

Exp Ther Med. 2021 Sep;22(3):943. doi: 10.3892/etm.2021.10375. Epub 2021 Jul 1.

Authors

Qiang Guo^{1

2}, Haijun Wang³, Yanzhao Xu¹, Mingbo Wang¹, Ziqiang Tian¹

Affiliations

¹ Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.
² Department of Thoracic Surgery, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
³ Department of Thoracic Surgery, Xingtai People's Hospital, Xingtai, Hebei 054000, P.R. China.

Abstract

Emerging studies have indicated that microRNAs (miRNAs/miRs) are involved in regulating non-small cell lung cancer (NSCLC)-associated processes. The present study aimed to evaluate the biological roles of miR-374a-5p in NSCLC. Using reverse transcription-quantitative PCR, the expression levels of miR-374a-5p were determined in NSCLC cells and a normal cell line. Functional experiments were performed to investigate the functions of miR-374a-5p in NSCLC. A luciferase activity reporter assay and rescue experiments were performed to validate NCK adaptor protein 1 (NCK1) as a functional target of miR-374a-5p. It was demonstrated that miR-374a-5p levels were decreased in NSCLC cell lines compared with those in a normal cell line. Furthermore, overexpression of miR-374a-5p inhibited NSCLC cell proliferation and migration in vitro. Of note, NCK1 overexpression reversed the effects of miR-375a-5p on NSCLC cell proliferation and migration. The present results confirmed the tumor suppressor role of miR-374a-5p via targeting NCK1 in NSCLC, indicating the importance of the miR-374a-5p/NCK1 axis in NSCLC.

Keywords: NCK adaptor protein 1; microRNA-374a-5p; non-small cell lung cancer; tumor suppressor.

Grants and funding

Funding: This project was supported by Pre-clinical Transformation Research of panel, a Multigene Molecular Marker for Early Diagnosis of Lung Adenocarcinoma (‘Office-School Consultation Fund-Science and Technology Innovation’ Project of Hebei Science and Technology Department-Hebei Medical University; grant. no. 2020TXZH04).