Combination of untargeted metabolomics approach and molecular networking analysis to identify unique natural components in wild Morchella sp. by UPLC-Q-TOF-MS

Xiaoyan Zhao; E Hengchao; Hui Dong; Yanmei Zhang; Jing Qiu; Yongzhong Qian; Changyan Zhou

doi:10.1016/j.foodchem.2021.130642

Combination of untargeted metabolomics approach and molecular networking analysis to identify unique natural components in wild Morchella sp. by UPLC-Q-TOF-MS

Food Chem. 2022 Jan 1:366:130642. doi: 10.1016/j.foodchem.2021.130642. Epub 2021 Jul 20.

Authors

Xiaoyan Zhao¹, E Hengchao², Hui Dong², Yanmei Zhang², Jing Qiu³, Yongzhong Qian⁴, Changyan Zhou⁵

Affiliations

¹ Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, China; Institute for Agro-food Standards and Testing Technology, Laboratory of Quality and Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai Academy of Agricultural Sciences, 1000 Jingqi Road, Shanghai 201403, China.
² Institute for Agro-food Standards and Testing Technology, Laboratory of Quality and Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai Academy of Agricultural Sciences, 1000 Jingqi Road, Shanghai 201403, China.
³ Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, China.
⁴ Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, China. Electronic address: qianyongzhong@caas.cn.
⁵ Institute for Agro-food Standards and Testing Technology, Laboratory of Quality and Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai Academy of Agricultural Sciences, 1000 Jingqi Road, Shanghai 201403, China. Electronic address: zhouchangyan@saaa.sh.cn.

PMID: 34304135
DOI: 10.1016/j.foodchem.2021.130642

Abstract

Morchella sp. is a typical edible fungus. However, its unique natural products, especially bioactive composition, are rarely reported. In this study, an untargeted metabolomics approach combined with multivariate statistical analysis was employed to screen the differential metabolites in Morchella sp. compared to four other edible fungi. Among the screened 50-top differential metabolites, 18 compounds involving fatty acids and peptides showed higher content in Morchella sp.. A unique peptide of desferriferricrocin was identified. Subsequently, molecular networking analysis revealed three novel homologous compounds of desferriferricrocin, which were annotated as desferriferrichrome, desferriferrichrome C, and a new homolog not previously reported. These results were further verified by quantitative analysis. To the best of our knowledge, this is the first report of four peptides in Morchella sp..

Keywords: Hydroxamate siderophore compounds; Molecular networking analysis; Morchella sp; UPLC-Q-TOF-MS; Untargeted metabolomics approach.

MeSH terms

Ascomycota*
Chromatography, High Pressure Liquid
Metabolomics*