Plant mitochondria import DNA via alternative membrane complexes involving various VDAC isoforms

Abstract

Mitochondria possess transport mechanisms for import of RNA and DNA. Based on import into isolated Solanum tuberosum mitochondria in the presence of competitors, inhibitors or effectors, we show that DNA fragments of different size classes are taken up into plant organelles through distinct channels. Alternative channels can also be activated according to the amount of DNA substrate of a given size class. Analyses of Arabidopsis thaliana knockout lines pointed out a differential involvement of individual voltage-dependent anion channel (VDAC) isoforms in the formation of alternative channels. We propose several outer and inner membrane proteins as VDAC partners in these pathways.

Keywords: Adenine nucleotide translocase; Arabidopsis thaliana; DNA import; Plant mitochondria; Protoplasts; Solanum tuberosum; Voltage-dependent anion channel isoforms.