RNA-Sequencing Analysis of Differentially Expressed Genes in Human iPSC-Derived Cardiomyocytes

Chikako Okubo; Megumi Narita; Takuya Yamamoto; Yoshinori Yoshida

doi:10.1007/978-1-0716-1484-6_19

RNA-Sequencing Analysis of Differentially Expressed Genes in Human iPSC-Derived Cardiomyocytes

Methods Mol Biol. 2021:2320:193-217. doi: 10.1007/978-1-0716-1484-6_19.

Authors

Chikako Okubo¹, Megumi Narita², Takuya Yamamoto^{3

4

5

6}, Yoshinori Yoshida⁷

Affiliations

¹ Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan. chikako.okubo@cira.kyoto-u.ac.jp.
² Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
³ Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan. takuya@cira.kyoto-u.ac.jp.
⁴ Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University, Kyoto, Japan. takuya@cira.kyoto-u.ac.jp.
⁵ Medical-Risk Avoidance Based on iPS Cells Team, RIKEN Center for Advanced Intelligence Project (AIP), Kyoto, Japan. takuya@cira.kyoto-u.ac.jp.
⁶ AMED-CREST, Tokyo, Japan. takuya@cira.kyoto-u.ac.jp.
⁷ Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. yoshinor@cira.kyoto-u.ac.jp.

PMID: 34302660
DOI: 10.1007/978-1-0716-1484-6_19

Abstract

RNA sequencing profiles and characterizes cell and tissue samples, giving important insights into molecular mechanisms. Such data is imperative for cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) and used in related translational and basic research. Here we provide reliable protocols to extract differentially expressed genes in iPSC-CMs with RNA sequencing.

Keywords: Gene expression; Genome-wide analysis; Molecular biology; Next-generation sequencer; RNA sequencing; Transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / genetics
Cells, Cultured
Chromosome Mapping
Gene Expression Profiling
Gene Library
Genes, Reporter
Genome, Human
Humans
Induced Pluripotent Stem Cells / cytology*
MicroRNAs / genetics
Myocytes, Cardiac / chemistry*
Principal Component Analysis
RNA / isolation & purification
RNA-Seq / methods*
Sequence Alignment
Software
Transcriptome*

Substances

MicroRNAs
RNA