Electrophysiological Analysis of hiPSC-Derived Cardiomyocytes Using a Patch-Clamp Technique

Methods Mol Biol. 2021:2320:121-133. doi: 10.1007/978-1-0716-1484-6_13.

Abstract

Electrophysiological analysis of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) using a patch-clamp technique enables the most precise evaluation of electrophysiological properties in single cells. Compared to multielectrode array (MEA) and membrane voltage imaging, patch-clamp recordings offer quantitative measurements of action potentials, and the relevant ionic currents which are essential for the research of disease modeling of inherited arrhythmias, safety pharmacology, and drug discovery using hiPSC-CMs. In this chapter, we describe the detail flow of patch-clamp recordings in hiPSC-CMs.

Keywords: Action potential recording; Electrophysiology; Human-induced pluripotent stem cell-derived cardiomyocyte; Patch-clamp technique; Voltage clamp.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / physiology
  • Arrhythmias, Cardiac / therapy
  • Cells, Cultured
  • Electrophysiological Phenomena / physiology*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Myocytes, Cardiac / cytology*
  • Patch-Clamp Techniques / methods*
  • Tissue Array Analysis / methods*