Multielectrode Array Assays Using Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Daisuke Yoshinaga; Yimin Wuriyanghai; Takeru Makiyama

doi:10.1007/978-1-0716-1484-6_12

Multielectrode Array Assays Using Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Methods Mol Biol. 2021:2320:111-119. doi: 10.1007/978-1-0716-1484-6_12.

Authors

Daisuke Yoshinaga^#¹, Yimin Wuriyanghai^#², Takeru Makiyama³

Affiliations

¹ Department of Pediatrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
² Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan.
³ Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan. makiyama@kuhp.kyoto-u.ac.jp.

^# Contributed equally.

PMID: 34302653
DOI: 10.1007/978-1-0716-1484-6_12

Abstract

Induced pluripotent stem cell (iPSC)-derived cardiomyocytes (iPSC-CMs) have been shown to have great potential to play a key role in investigating cardiac diseases in vitro. Multielectrode array (MEA) system is sometimes preferable to patch-clamp in electrophysiological experiments in terms of several advantages. Here we show our protocol of electrophysiological examinations using MEA.

Keywords: Electrophysiology; Extracellular recording; Field potential duration; Human-induced pluripotent stem cell-derived cardiomyocyte; Multielectrode array.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Assay / methods*
Cells, Cultured
Electrophysiological Phenomena / physiology
Humans
Induced Pluripotent Stem Cells / cytology*
Myocytes, Cardiac / cytology*
Patch-Clamp Techniques / methods
Tissue Array Analysis / methods*