Protocol for Morphological and Functional Phenotype Analysis of hiPS-Derived Cardiomyocytes

Jun Li; Jong-Kook Lee

doi:10.1007/978-1-0716-1484-6_10

Protocol for Morphological and Functional Phenotype Analysis of hiPS-Derived Cardiomyocytes

Methods Mol Biol. 2021:2320:91-100. doi: 10.1007/978-1-0716-1484-6_10.

Authors

Jun Li¹, Jong-Kook Lee²

Affiliations

¹ Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, Suita, Japan.
² Department of Cardiovascular Regenerative Medicine, Osaka University Graduate School of Medicine, Suita, Japan. jlee@cardiology.med.osaka-u.ac.jp.

PMID: 34302651
DOI: 10.1007/978-1-0716-1484-6_10

Abstract

Induced pluripotent stem cells (iPSCs) have been utilized to study physiological development and also the pathogenesis of heart diseases. iPS-derived cardiomyocytes and engineered cardiac tissues provide a promising capacity for investigating cardiac development and disease modeling. In addition to protocols for cardiac differentiation and 3D cardiac tissue construction, the establishment of protocols for the comprehensive evaluation of the physiological and/or pathophysiological properties for the iPS-derived cells/tissues are indispensable.

Keywords: Calcium transient; Cardiac conduction; Contraction; Membrane potential; Morphology; iPS-derived cardiomyocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / physiology
Cells, Cultured
Heart Diseases / therapy
Humans
Induced Pluripotent Stem Cells / cytology*
Myocytes, Cardiac / cytology*
Phenotype
Tissue Engineering / methods