Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature

Barry G H Janssen; Mohamadreza Najiminaini; Yan Min Zhang; Parsa Omidi; Jeffrey J L Carson

doi:10.1186/s42649-021-00059-6

Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature

Appl Microsc. 2021 Jul 24;51(1):12. doi: 10.1186/s42649-021-00059-6.

Authors

Barry G H Janssen^{1

2}, Mohamadreza Najiminaini^{3

4}, Yan Min Zhang^{5

6

7

8}, Parsa Omidi^{3

4

8}, Jeffrey J L Carson^{9

3

4

8}

Affiliations

¹ Department of Medical Biophysics, Western University, London, ON, N6A 5C1, Canada. gjansse@uwo.ca.
² Kidney Clinical Research Unit (KCRU), London Health Sciences Centre, London, ON, N6C 6B5, Canada. gjansse@uwo.ca.
³ Imaging Program, St.Joseph's Health Care, Lawson Health Research Institute, London, ON, N6A 4V2, Canada.
⁴ Department of Pathology, Western University, London, ON, N6A 5C1, Canada.
⁵ Kidney Clinical Research Unit (KCRU), London Health Sciences Centre, London, ON, N6C 6B5, Canada.
⁶ Trauma Research Centre, Fourth Medical Center of the Chinese PLA General Hospital, Beijing, 100048, People's Republic of China.
⁷ School of Biomedical Engineering, Western University, London, ON, N6A 3K7, Canada.
⁸ Intensive Care Unit, Tianjin Nankai Hospital, Tianjin, 300100, People's Republic of China.
⁹ Department of Medical Biophysics, Western University, London, ON, N6A 5C1, Canada.

Abstract

Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation.

Keywords: Brightfield microscopy; Fluorescence microscopy; Intravital microscopy; Multispectral microscopy.

Abstract

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