Hemocompatibility, biodegradability and acute toxicity of acetylated cellulose nanocrystals of different types in comparison

Abstract

Promotion of promising cellulose nanocrystals (CNC) is largely dependent on the relationship between their morphology, surface chemical composition, and supramolecular structure with toxicity, hemocompatibility, and biodegradability. This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II. These data have also included the study of CNC obtained from the sulfuric acid solutions. The aqueous solution of all types of tested CNC has not been toxic to mice after oral administration. Morphology of internal organs has not changed. However, in case of disc-like particles, the kidney mass coefficient noticeably changed. CNC have neither triggered platelet aggregation nor destroyed the red cell membrane. Intravenous administration to rabbits has not affected the plasma clotting time. Rod-like CNC are more resistant, and the disc-like particles are more susceptible to degradation under the influence of cellulases.

Keywords: Acute toxicity; Cellulose allotropes; Cellulose nanocrystals; Enzymatic destruction; In vitro/ex vivo hemocompatibility.