Precise control of blood clotting and rapid reversal of anticoagulation are essential in many clinical situations. We were successful in modifying a thrombin-binding aptamer with a red-light photocleavable linker derived from Cy7 by Cu-catalyzed Click chemistry. We were able to show that we can successfully deactivate the modified aptamer with red light (660 nm) even in human blood-restoring the blood's natural coagulation capability.
Keywords: aptamer; oligonucleotide; photocaging; phototherapeutic window; red light.
© 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.