Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides

Lequn Geng; Jiaqi Shen; Wenjing Wang

doi:10.1039/d1cc02643g

Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides

Chem Commun (Camb). 2021 Aug 12;57(65):8051-8054. doi: 10.1039/d1cc02643g.

Authors

Lequn Geng¹, Jiaqi Shen¹, Wenjing Wang¹

Affiliation

¹ Life Sciences Institute, University of Michigan, Ann Arbor, Michigan, USA. wenjwang@umich.edu and Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA.

PMID: 34291777
DOI: 10.1039/d1cc02643g

Abstract

We re-engineered a commonly-used light-sensing protein, AsLOV2, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide. We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging. In summary, circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.