Development of edible Thai rice film fortified with ginger extract using microwave-assisted extraction for oral antimicrobial properties

Sci Rep. 2021 Jul 21;11(1):14870. doi: 10.1038/s41598-021-94430-y.

Abstract

This study aimed to investigate microwave-assisted extraction (MAE) of dried ginger and to develop a rice-based edible film incorporating ginger extract. The optimal MAE conditions of 400 W microwave power and an extraction time of 1 min were determined using a 32 full factorial design. The optimized extract showed total phenolic compounds (TPC, 198.2 ± 0.7 mg gallic acid equivalent/g), antioxidant activity measured by DPPH (91.4 ± 0.6% inhibition), ABTS (106.4 ± 3.1 mg Trolox/g), and FRAP (304.6 ± 5.5 mg Trolox/g), and bioactive compounds including 6-gingerol (71.5 ± 3.6 mg/g), 6-shogaol (12.5 ± 1.0 mg/g), paradol (23.1 ± 1.1 mg/g), and zingerone (5.0 ± 0.3 mg/g). Crude extract of dried ginger showed antimicrobial activity against Streptococcus mutans DMST 18777, with a minimum inhibitory concentration and minimum bactericidal concentration of 0.5 and 31.2 mg/mL, respectively. The rice-based edible film incorporating 3.2% (w/v) ginger extract tested against S. mutans DMST 18777 had a mean zone of inhibition of 12.7 ± 0.1 mm. Four main phenolic compounds, 6-gingerol, 6-shogaol, paradol, and zingerone, and six volatile compounds, α-curcumene, α-zingiberene, γ-muurolene, α-farnesene, β-bisabolene, and β-sesquiphellandrene, were found in rice film fortified with crude ginger extract.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catechols / isolation & purification
  • Catechols / pharmacology*
  • Drug Resistance, Bacterial
  • Edible Films*
  • Fatty Alcohols / isolation & purification
  • Fatty Alcohols / pharmacology*
  • Guaiacol / analogs & derivatives*
  • Guaiacol / isolation & purification
  • Guaiacol / pharmacology
  • Microwaves*
  • Oryza / chemistry*
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Solid Phase Extraction / methods*
  • Streptococcus mutans / drug effects*
  • Thailand
  • Zingiber officinale / chemistry*

Substances

  • Catechols
  • Fatty Alcohols
  • Plant Extracts
  • zingerone
  • Guaiacol
  • shogaol
  • gingerol