Determination of Intracellular Osmolytes in Cyanobacterial Cells

Xiaoming Tan; Kuo Song; Cuncun Qiao; Xuefeng Lu

doi:10.21769/BioProtoc.2812

Determination of Intracellular Osmolytes in Cyanobacterial Cells

Bio Protoc. 2018 Apr 20;8(8):e2812. doi: 10.21769/BioProtoc.2812.

Authors

Xiaoming Tan¹, Kuo Song¹, Cuncun Qiao¹, Xuefeng Lu^{1

2}

Affiliations

¹ Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.
² Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.

Abstract

Most of the cyanobacteria accumulate osmolytes including sucrose, glucosylglycerol, in their cells in response to salt stress. Here we describe a protocol of our laboratory for extraction and quantification of cyanobacterial intracellular sucrose and glucosylglycerol. We have confirmed this protocol was applicable to at least four kinds of cyanobacteria, filamentous cyanobacterium Anabaena sp. PCC 7120, unicellular cyanobacterium Synechocystis sp. PCC 6803, Synechococcus elongatus PCC 7942 and halotolerant unicellular cyanobacterium Synechococcus sp. PCC 7002.

Keywords: Cyanobacteria; Glucosylglycerol; Ion chromatography; Osmolyte; Sucrose; Synechocystis.