Evaluation of Root pH Change Through Gel Containing pH-sensitive Indicator Bromocresol Purple

Aparecida L Silva; Keini Dressano; Paulo H O Ceciliato; Juan Carlos Guerrero-Abad; Daniel S Moura

doi:10.21769/BioProtoc.2796

Evaluation of Root pH Change Through Gel Containing pH-sensitive Indicator Bromocresol Purple

Bio Protoc. 2018 Apr 5;8(7):e2796. doi: 10.21769/BioProtoc.2796.

Authors

Aparecida L Silva¹, Keini Dressano¹, Paulo H O Ceciliato¹, Juan Carlos Guerrero-Abad¹, Daniel S Moura¹

Affiliation

¹ Laboratório de Bioquímica de Proteínas, Departamento de Ciências Biológicas, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo (ESALQ/USP), Piracicaba, SP, Brazil.

Abstract

The Rapid Alkalinization Factor (RALF) is a plant hormone peptide that inhibits proton transport causing alkalinization of the extracellular media. To detect the alkalinization response elicited by RALF peptides in root cells, Arabidopsis seedlings are carefully transferred to a gel containing the pH-sensitive indicator bromocresol purple, treated with the peptide and photographed after 30 min. Herein the protocol is optimized for evaluation of exogenous treatment, described in detail and expected results are presented.

Keywords: Alkalinization; Bromocresol purple; RALF; pH indicator.