Enzymatic Activity Assay for Invertase in Synechocystis Cells

Xiaoming Tan; Kuo Song; Xuefeng Lu

doi:10.21769/BioProtoc.2856

Enzymatic Activity Assay for Invertase in Synechocystis Cells

Bio Protoc. 2018 May 20;8(10):e2856. doi: 10.21769/BioProtoc.2856.

Authors

Xiaoming Tan¹, Kuo Song¹, Xuefeng Lu^{1

2}

Affiliations

¹ Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.
² Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.

Abstract

Invertase can catalyze the hydrolysis of sucrose, and is widely distributed in cells of cyanobacteria and plants. Being responsible for the first step for sucrose metabolism, invertase plays important physiological roles and its enzymatic activity is frequently needed to be determined. All the methods for determination of the invertase activity are dependent on detection of the glucose product generated by the invertase. Here we describe an ion chromatography based protocol of our laboratory for determination of cyanobacterial intracellular invertase activity.

Keywords: Cyanobacteria; Invertase enzymatic activity; Ion chromatography; Sucrose; Synechocystis.