Comprehensive serum and tissue microRNA profiling in dedifferentiated liposarcoma

Isaku Kohama; Naofumi Asano; Juntaro Matsuzaki; Yusuke Yamamoto; Tomofumi Yamamoto; Ryou-U Takahashi; Eisuke Kobayashi; Satoko Takizawa; Hiromi Sakamoto; Ken Kato; Hiroyuki Fujimoto; Hirotaka Chikuda; Akira Kawai; Takahiro Ochiya

doi:10.3892/ol.2021.12884

Comprehensive serum and tissue microRNA profiling in dedifferentiated liposarcoma

Oncol Lett. 2021 Aug;22(2):623. doi: 10.3892/ol.2021.12884. Epub 2021 Jun 29.

Authors

Isaku Kohama^{1

2}, Naofumi Asano³, Juntaro Matsuzaki¹, Yusuke Yamamoto¹, Tomofumi Yamamoto¹, Ryou-U Takahashi⁴, Eisuke Kobayashi⁵, Satoko Takizawa⁶, Hiromi Sakamoto⁷, Ken Kato⁸, Hiroyuki Fujimoto⁹, Hirotaka Chikuda², Akira Kawai⁵, Takahiro Ochiya^{1

10}

Affiliations

¹ Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
² Department of Orthopedic Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
³ Department of Orthopedic Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan.
⁴ Department of Cellular and Molecular Biology, Division of Integrated Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
⁵ Department of Musculoskeletal Oncology, National Cancer Center Hospital, Tokyo 104-0045, Japan.
⁶ New Frontiers Research Institute, Toray Industries, Kamakura, Kanagawa 247-8555, Japan.
⁷ Department of Clinical Genomics, Fundamental Innovative Oncology Core, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
⁸ Department of Gastrointestinal Medical Oncology, National Cancer Center Hospital, Tokyo 104-0045, Japan.
⁹ Department of Urology, National Cancer Center Hospital, Tokyo 104-0045, Japan.
¹⁰ Department of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University, Tokyo 160-0023, Japan.

Abstract

Sarcoma is a rare cancer with several subtypes; therefore, our understanding of the pathogenesis of sarcoma is limited, and designing effective treatments is difficult. Circulating microRNAs (miRNAs), including exosomal miRNAs, have attracted attention as biomarkers in cancer. However, the roles of miRNAs and exosomes in sarcoma remain unclear. The present analysis of tissue and serum miRNA expression in osteosarcoma, Ewing's sarcoma and dedifferentiated liposarcoma (DDLPS) identified miR-1246, -4532, -4454, -619-5p and -6126 as biomarkers for DDLPS. These miRNAs were highly expressed in human DDLPS cell lines and exosomes, suggesting that they are secreted from DDLPS tissues. The present results suggested that specific miRNAs may be used as biomarkers for early diagnosis or treatment targets in DDLPS.

Keywords: cancer; exosome; liquid biopsy; microRNA; sarcoma.

Grants and funding

The study was funded by The National Cancer Center Research and Development Fund (grant no. 29-A-1) and through a Development of Diagnostic Technology for Detection of miRNA in Body Fluids grant from the Japan Agency for Medical Research and Development.