Review of Infections With Bovine Herpesvirus 1 in Slovenia

Peter Hostnik; Danijela Černe; Janko Mrkun; Jože Starič; Ivan Toplak

doi:10.3389/fvets.2021.676549

Review of Infections With Bovine Herpesvirus 1 in Slovenia

Front Vet Sci. 2021 Jul 1:8:676549. doi: 10.3389/fvets.2021.676549. eCollection 2021.

Authors

Peter Hostnik¹, Danijela Černe¹, Janko Mrkun², Jože Starič³, Ivan Toplak¹

Affiliations

¹ Institute for Microbiology and Parasitology-Virology Unit, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.
² Clinic for Reproduction and Large Animals-Clinic for Reproduction, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.
³ Clinic for Reproduction and Large Animals-Section for Ruminants, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.

Abstract

In the 1950s, infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV) disease was clinically detected and documented in cattle for the first time in Slovenia. The bovine herpes virus 1 (BoHV-1) was confirmed several times from infected herds by virus isolation on cell cultures. To keep the IC virus-free, high biosecurity measures were introduced. Before entering the IC, all calves are serologically tested and quarantined. Bulls in Slovenian insemination centres (IC) have been negative for IBR /IPV infection since 1979. From 1985 to 1991, few large-scale studies of the prevalence of IBR/IPV were carried out. In 1985, a high percentage (56.9%) of serologically positive animals were found in large state farms with Holstein Friesian cattle. Epidemiological studies in farm with bulls' mother herds were also carried out in the farms with Simmental and Brown cows. Antibodies against BoHV-1 were detected in the serum of 2.3% of Brown cattle and 3.5% of Simmental cattle. In the year 2000, 3.4% of bulk tank milk samples from 13,349 dairy farms were detected BoHV-1 antibodies positive. The highest percentage of positive animals was found in regions with an intensive grazing system (6.2% positive) and the lowest percentage in the east part of Slovenia (0.9% positive) on farms with mostly Simmental cattle. In 2006, a total 204,662 sera of cattle older than 24 months were tested for the presence of BoHV-1 antibodies and positive cattle were detected in 3.6% of tested farms. These farms kept 34,537 animals that were potential carriers of the BoHV-1. Most of the positive farms kept Holstein Friesian cattle, descendants from the state-owned farms, which were privatised or closed after 1990. In 2015, the Administration of the Republic of Slovenia for Food Safety, Veterinary and Plant Protection issued a rule that describes the conditions for granting and maintaining the status of BoHV-1 free holdings. The rule provides a voluntary control programme for breeders who want to obtain BoHV-1 free status and are willing to cover all the cost of acquiring and maintaining that status. There has been very little response from breeders.

Keywords: BoHV-1; Slovenia; antibody detection; insemination centres; monitoring.