Structure and genetics of the O-antigen of Enterobacter cloacae K7 containing di-N-acetylpseudaminic acid

Andrei V Filatov; Andrei V Perepelov; Alexander S Shashkov; Gennady L Burygin; Natalia E Gogoleva; Yuriy A Khlopko; Vyacheslav S Grinev

doi:10.1016/j.carres.2021.108392

Structure and genetics of the O-antigen of Enterobacter cloacae K7 containing di-N-acetylpseudaminic acid

Carbohydr Res. 2021 Oct:508:108392. doi: 10.1016/j.carres.2021.108392. Epub 2021 Jul 6.

Authors

Andrei V Filatov¹, Andrei V Perepelov², Alexander S Shashkov², Gennady L Burygin³, Natalia E Gogoleva⁴, Yuriy A Khlopko⁵, Vyacheslav S Grinev⁶

Affiliations

¹ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation. Electronic address: filatov_andrey_@mail.ru.
² N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
³ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 410049, Saratov, Russian Federation; Vavilov Saratov State Agrarian University, 410012, Saratov, Russian Federation.
⁴ Kazan Institute of Biochemistry and Biophysics, Kazan Science Centre, Russian Academy of Sciences, 420111, Kazan, Russian Federation; Kazan Federal University, 420111, Kazan, Russian Federation.
⁵ Institute for Cellular and Intracellular Symbiosis, Urals Branch, Russian Academy of Sciences, 460000, Orenburg, Russian Federation.
⁶ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 410049, Saratov, Russian Federation.

PMID: 34274818
DOI: 10.1016/j.carres.2021.108392

Abstract

The O-antigen (O-polysaccharide) is an essential component of lipopolysaccharide on the surface of Gram-negative bacteria and plays an important role in interaction with host organisms. In this study, we investigated the chemical structure and characterized the gene cluster of Enterobacter cloacae K7 O-antigen. As judged by sugar analyses along with NMR spectroscopy data, E. cloacae K7 antigen has a tetrasaccharide O-unit with the following structure: →8)-β-Psep5Ac7Ac-(2 → 2)-β-l-Rhap-(1 → 4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1→ The O-antigen gene cluster of E. cloacae K7 between conserved genes galF and gnd was sequenced. Most genes necessary for the O-antigen synthesis were found in the cluster and their functions were tentatively assigned by comparison with sequences in the available databases.

Keywords: Bacterial polysaccharide structure; Enterobactercloacae; O-antigen; O-antigen gene cluster; Pseudaminic acid.

MeSH terms

Multigene Family
O Antigens*
Sialic Acids*

Substances

5,7-diacetamido-3,5,7,9-tetradeoxynon-2-ulosonic acid
O Antigens
Sialic Acids