One wavelength to excite them all: deep tissue imaging going multicolor

Cristina Rodríguez; Na Ji

doi:10.1016/j.tins.2021.07.001

One wavelength to excite them all: deep tissue imaging going multicolor

Trends Neurosci. 2021 Sep;44(9):689-691. doi: 10.1016/j.tins.2021.07.001. Epub 2021 Jul 14.

Authors

Cristina Rodríguez¹, Na Ji²

Affiliations

¹ Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. Electronic address: crisrod@berkeley.edu.
² Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA; Department of Physics, University of California, Berkeley, CA, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, CA, USA; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.

PMID: 34274148
DOI: 10.1016/j.tins.2021.07.001

Abstract

Three-photon fluorescence microscopy has emerged as one of the leading tools for deep tissue imaging in opaque biological specimens. A recent study by Hontani et al. demonstrated a new strategy for multicolor imaging deep in the mouse brain using a single excitation wavelength.

Keywords: brain; calcium imaging; glia; neural circuits; neurovascular; three-photon fluorescence imaging.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Comment

MeSH terms

Animals
Brain / cytology*
Mice
Microscopy, Fluorescence, Multiphoton*

Grants and funding

U01 NS118300/NS/NINDS NIH HHS/United States