A simple and rapid fluorescent approach for Pb2+ determination and application in water samples and living cells

Chunyu Jiang; Liu Yang; Ping Li; Yulong Liu; Shijie Li; Ying Fu; Fei Ye

doi:10.1016/j.saa.2021.120168

A simple and rapid fluorescent approach for Pb²⁺ determination and application in water samples and living cells

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Dec 15:263:120168. doi: 10.1016/j.saa.2021.120168. Epub 2021 Jul 10.

Authors

Chunyu Jiang¹, Liu Yang¹, Ping Li¹, Yulong Liu¹, Shijie Li², Ying Fu³, Fei Ye⁴

Affiliations

¹ Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
² College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
³ Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address: fuying@neau.edu.cn.
⁴ Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address: yefei@neau.edu.cn.

PMID: 34273892
DOI: 10.1016/j.saa.2021.120168

Abstract

A novel selective fluorescent chemosensor, thiosemicarbazide-appended naphthalimide derivative (TND), has been designed and synthesized, which exhibited good selectivity and sensibility for Pb²⁺ in CH₃CN:H₂O (1:1) solution. The probe TND showed obvious color changes under UV light of 365 nm and displayed turn-on fluorescence response with Pb²⁺ added. The binding mode of TND with Pb²⁺ was found to be 1:1 based on the Job's plot analysis. The detection limit of Pb²⁺ was 4.7 nM, which is far below the allowable concentration determined by WHO in drinking water. Moreover, the fortified recoveries of Pb²⁺ were from 100.54% to 113.68% in water samples. TND is also applied for fluorescence imaging of Pb²⁺ in lysosomes of human stromal cell line (HSC). This study indicated that TND would be a potential sensor detecting Pb²⁺ in real sample.

Keywords: Fluorescent probe; Pb(2+); Target lysosomes; Thiosemicarbazide-appended 1,8-naphthalimide; Water samples.

MeSH terms

Fluorescent Dyes*
Humans
Lead*
Naphthalimides
Spectrometry, Fluorescence
Water

Substances

Fluorescent Dyes
Naphthalimides
Water
Lead