Caspase-3 and gasdermin E detection in peri-implantitis

Chaozhen Chen; Zhiwei Jiang; Qifeng Jiang; Wei Dai; Qin Shao; Qianming Chen; Ying Wang; Guoli Yang

doi:10.1016/j.bbadis.2021.166217

Caspase-3 and gasdermin E detection in peri-implantitis

Biochim Biophys Acta Mol Basis Dis. 2021 Nov 1;1867(11):166217. doi: 10.1016/j.bbadis.2021.166217. Epub 2021 Jul 14.

Authors

Chaozhen Chen¹, Zhiwei Jiang¹, Qifeng Jiang¹, Wei Dai¹, Qin Shao¹, Qianming Chen¹, Ying Wang², Guoli Yang³

Affiliations

¹ Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Clinical Research Center for Oral diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou 310006, China.
² Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Clinical Research Center for Oral diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou 310006, China. Electronic address: 7314032@zju.edu.cn.
³ Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Clinical Research Center for Oral diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou 310006, China. Electronic address: guo_li1977@zju.edu.cn.

PMID: 34273529
DOI: 10.1016/j.bbadis.2021.166217

Abstract

Peri-implantitis could lead to progressive bone loss and implant failure; however, the mechanism of peri-implantitis remains unclear. Based on emerging evidence, pyroptosis, a novel proinflammatory programmed death, contributes to different oral infectious diseases. In the present study, we investigated the involvement of cleaved caspase-3 and gasdermin E (GSDME) in peri-implantitis and established a pyroptosis model in vitro. By collecting and examining the inflamed biopsies around peri-implantitis, we found that the pyroptosis-related markers (caspase-3, GSDME, and IL-1β) were enhanced relative to levels in control individuals. Furthermore, human gingival epithelium cells (HGECs) induced by tumor necrosis factor-α (TNF-α) exhibited pyroptosis morphological changes (cell swelling and balloon-shaped bubbles) and upregulated expression of pyroptosis-related markers. Pretreated with Ac-DEVD-CHO (a caspase-3 inhibitor) or GSDME small interference RNA (siRNA) were found to attenuate pyroptosis in HGECs. In conclusion, our findings revealed a high expression of caspase-3 and GSDME in the inflamed biopsies of peri-implantitis and confirmed that the caspase-3/GSDME pathway mediates TNF-α-triggered pyroptosis in human gingival epithelium cells, which provides a new target for peri-implantitis treatment.

Keywords: Human gingival epithelium cell; Implant; Pyroptotic cell death; Soft tissue seal.

Publication types

Observational Study
Research Support, Non-U.S. Gov't

MeSH terms

Biopsy
Case-Control Studies
Caspase 3 / analysis
Caspase 3 / metabolism*
Cell Line
Epithelial Cells
Gingiva / immunology
Gingiva / pathology*
Healthy Volunteers
Humans
Mouth Mucosa / immunology
Mouth Mucosa / pathology*
Peri-Implantitis / immunology*
Peri-Implantitis / pathology
Pyroptosis / immunology
Receptors, Estrogen / analysis
Receptors, Estrogen / metabolism*

Substances

GSDME protein, human
Receptors, Estrogen
CASP3 protein, human
Caspase 3