In Vitro Anther Culture for Doubled Haploid Plant Production in Spelt Wheat

Methods Mol Biol. 2021:2287:257-266. doi: 10.1007/978-1-0716-1315-3_13.

Abstract

Doubled haploid (DH) plant production belongs to modern biotechnology methods of plant breeding. The main advantage of DH plant production methods is the development of genetically homozygous lines in one generation, whilst in conventional breeding programmes, the development of homozygous lines requires more generations. The present chapter describes an efficient protocol for DH plant production in spelt wheat genotypes using in vitro anther culture.

Keywords: Anther culture; Doubled haploid; In vitro androgenesis; Spelt wheat.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Flowers / genetics
  • Flowers / growth & development*
  • Haploidy
  • In Vitro Techniques / methods
  • Plant Breeding / methods*
  • Pollen / genetics
  • Pollen / growth & development
  • Tissue Culture Techniques / methods*
  • Triticum / genetics
  • Triticum / growth & development*