Purpose: The purpose of this study was to detect the differentially expressed microRNA-186-5p in cervical cancer samples and to uncover its influence on the development of cervical cancer.
Methods: Differentially expressed microRNA-186-5p was detected in cervical cancer tissues and normal cervical tissues. Its influences on clinical features and prognosis of cervical cancer patients were analyzed. Regulatory effect of microRNA-186-5p on migratory potential in HeLa and C33-A cells was examined. In addition, the target gene binding to microRNA-186-5p was searched and its involvement in the malignant development of cervical cancer was determined.
Results: MicroRNA-186-5p was downregulated in cervical cancer patients, especially those with lymphatic metastasis or distant metastasis. High metastasis rate and poor prognosis were seen in cervical cancer patients expressing a low level of microRNA-186-5p. Overexpression of microRNA-186-5p inhibited migratory ability of cervical cancer cells. FZD3 was the downstream gene binding to microRNA-186-5p, which was upregulated in cervical cancer samples. Besides, it was capable of reversing the role of microRNA-186-5p in inhibiting migratory ability of cervical cancer cells.
Conclusions: MicroRNA-186-5p is lowly expressed in cervical cancer samples, and it inhibits the metastasis in cervical cancer cells by targeting FZD3.