In recent years, wheat germ lipase (WGL) is attracting considerable interest. To date, several WGL applications have already been described: (i) fats and oils modification; (ii) esterification reactions in organic media, accepting a wide range of acids and alcohols as substrates; (iii) the asymmetric resolution of various chiral racemic intermediates; (iv) more recently, the promiscuous activity of WGL has been shown in carbon-carbon bond formation. To date, no crystallographic structure of this enzyme has been published, which means its activity, catalytic potential and substrate scope is being assessed empirically. Therefore, new catalytic activities of this enzyme are constantly being discovered. Taking into account the emergency and the current interest in environmentally sustainable processes, this review aims to highlight the origin, isolation, stabilization by immobilization and applications of the wheat germ lipase.HIGHLIGHTSWheat germ as an inexpensive source of biocatalystsWheat germ lipase an efficient catalyst for various chemical transformationsWheat germ lipase in food productionIndustrial applications of wheat germ lipaseWheat germ lipase as a promiscuous biocatalystImmobilization of wheat germ lipase as a method of stabilization.
Keywords: Wheat germ lipase; enzymatic resolution; enzyme promiscuity; esterification; hydrolysis; immobilization.