Carbonized biosorbent assisted matrix solid-phase dispersion microextraction for active compounds from functional food

Abstract

In this study, mangosteen peel based activated carbon was prepared and first applied as adsorbent in matrix solid-phase dispersion (MSPD) for simultaneously extraction of flavonoids from Dendrobium huoshanense prior to their separation and determination by ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF/MS). The MSPD-UHPLC-Q-TOF/MS method was validated exhaustively. Good linearities (r² ≥ 0.9929) were obtained for all target analytes. The limits of detection was in the range of 0.00387-0.159 μg/g. Satisfactory recoveries of six target compounds were between 80.02 and 99.49% and 85.32-99.86% for the low and high spiked level, respectively. Furthermore, relative to other common sorbent, the prepared mangosteen peel based activated carbon was less expensive and more environmentally-friendly. Consequently, the proposed method was a simple, efficient, low-cost, eco-friendly, time-saving and sensitive approach that could be successfully applied to the extraction and determination of flavonoids compounds in complex matrix.

Keywords: Dendrobium huoshanense; Mangosteen peel based activated carbon; Matrix solid-phase dispersion; Quadrupole time-of-flight tandem mass spectrometry; Ultra-high performance liquid chromatography.