The differential inhibitive effects and fates of As(III) and As(V) mediated by Sulfobacillus thermosulfidooxidans grown on S0, Fe2+ and FeS2

Abstract

Arsenic often coexists with metal sulfide minerals and occurs in different speciation and different toxicity in responding to Fe/S biooxidation. The differential inhibitive effects and fates of As(III) and As(V) during biooxidations of elemental sulfur (S⁰), ferrous ions (Fe²⁺) and pyrite (FeS₂) by Sulfobacillus thermosulfidooxidans were studied. The results revealed that the arsenic species hardly changed for the biooxidation of S⁰, but dramatically changed for the biooxidation of Fe²⁺ and FeS₂. Different transformation degree between As(III) and As(V) occurred for biooxidation of FeS₂ in the presence of arsenic, where about 72% of As(III) was transformed to As(V) for the group with As(III) added, and 16% of As(V) was transformed to As(III) for that with As(V) added. Both formation and dissolution of amorphous ferric arsenate occurred during biooxidation of FeS₂ with the addition of As(III) or As(V) and for the group grown on Fe²⁺ with added As(V), which were controlled by the changes of Fe/As molar ratio and pH value in the solution. Jarosite was detected for the group grown on Fe²⁺ and could adsorb As(III) and As(V). The inhibitive effects of As(V) were higher than As(III) when the strain grew on FeS₂, which was contrary to those when the strain grew on S⁰ and Fe²⁺. The above results signify that the fates and inhibitive effects of arsenic are much related to each other, and such a relationship is significantly affected by the utilization of Fe/S energy substrates by the sulfur- and ferrous-oxidizing microorganisms.

Keywords: Arsenic ions; Arsenic transformation; Biooxidation; Inhibitive effects; Sulfobacillus thermosulfidooxidans.