Evaluation of Staphylococcus aureus Antibiotic Tolerance Using Kill Curve Assays

Jessica N Brandwein; Kelly C Rice

doi:10.1007/978-1-0716-1550-8_7

Evaluation of Staphylococcus aureus Antibiotic Tolerance Using Kill Curve Assays

Methods Mol Biol. 2021:2341:45-54. doi: 10.1007/978-1-0716-1550-8_7.

Authors

Jessica N Brandwein¹, Kelly C Rice²

Affiliations

¹ Department of Microbiology and Cell Science, IFAS, University of Florida, Gainesville, FL, USA.
² Department of Microbiology and Cell Science, IFAS, University of Florida, Gainesville, FL, USA. kcrice@ufl.edu.

PMID: 34264460
DOI: 10.1007/978-1-0716-1550-8_7

Abstract

This chapter describes the use of antibiotic kill curves to examine the tolerance of Staphylococcus aureus to any antibiotic of interest. This is done by treating cultures with a super-minimum inhibitory concentration (MIC) of antibiotic and measuring viability over time by colony-forming units (CFUs). Kill curves provide a unique insight into S. aureus antibiotic tolerance and death patterns that may not be clear from other experiments, such as traditional MIC or Kirby-Bauer assays.

Keywords: Antibiotic tolerance; CFU; Cell death; MIC; Persistence.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Anti-Bacterial Agents / pharmacology*
Colony Count, Microbial
Drug Resistance, Bacterial*
Microbial Sensitivity Tests
Microbial Viability / drug effects
Staphylococcus aureus / drug effects
Staphylococcus aureus / growth & development*

Substances

Anti-Bacterial Agents