Lactoferrin could alleviate liver injury caused by Maillard reaction products with furan ring through regulating necroptosis pathway

Linlin Fan; Fengen Wang; Qianqian Yao; Haoming Wu; Fang Wen; Jiaqi Wang; Huiying Li; Nan Zheng

doi:10.1002/fsn3.2254

Lactoferrin could alleviate liver injury caused by Maillard reaction products with furan ring through regulating necroptosis pathway

Food Sci Nutr. 2021 May 6;9(7):3449-3459. doi: 10.1002/fsn3.2254. eCollection 2021 Jul.

Authors

Linlin Fan^{1

2

3}, Fengen Wang^{1

2

3}, Qianqian Yao^{1

2

3}, Haoming Wu^{1

2

3}, Fang Wen^{1

2

3}, Jiaqi Wang^{1

2

3}, Huiying Li^{1

2

3}, Nan Zheng^{1

2

3}

Affiliations

¹ Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China.
² Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China.
³ State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China.

Abstract

As classical MRPs, the toxic effects of furosine, pyralline, and 5-hydroxymethylfurfural (5-HMF) in liver tissue are evaluated and the related mechanism is investigated here, and the protective effects of lactoferrin on liver injury caused by Maillard reaction products (MRPs) with furan ring are proved in vitro and in vivo. First, we detect the concentrations of furosine, pyralline, and 5-HMF in several foods using ultrahigh-performance liquid chromatography (UHPLC). Then, the effects of the three MRPs on liver cells (HL-7702) viability, as well as liver tissue, are performed and evaluated. Furthermore, the regulations of three MRPs on necroptosis-related pathway in liver cells are investigated. Additionally, the effects of lactoferrin in alleviating liver injury, as well as regulating necroptosis pathway, were evaluated. Results elucidate that lactoferrin protects liver injury caused by MRPs with furan ring structure through activating RIPK1/RIPK3/p-MLKL necroptosis pathway and downstream inflammatory reaction.

Keywords: 5‐hydroxymethylfurfural (5‐HMF); Maillard reaction products (MRPs); furosine; lactoferrin; pyralline.