Polyhexamethylene guanidine phosphate, chloromethylisothiazolinone, and particulate matter are dispensable for stress granule formation in human airway epithelial cells

Arnoldo Cambronero-Urena; Sunkyung Choi; Seri Choi; Kee K Kim; Eun-Mi Kim

doi:10.1080/19768354.2021.1931442

Polyhexamethylene guanidine phosphate, chloromethylisothiazolinone, and particulate matter are dispensable for stress granule formation in human airway epithelial cells

Anim Cells Syst (Seoul). 2021 May 26;25(3):146-151. doi: 10.1080/19768354.2021.1931442. eCollection 2021.

Authors

Arnoldo Cambronero-Urena¹, Sunkyung Choi¹, Seri Choi², Kee K Kim¹, Eun-Mi Kim²

Affiliations

¹ Department of Biochemistry, Chungnam National University, Daejeon, South Korea.
² Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon, South Korea.

Abstract

Environmental risk factors are recognized as threats to public health. Stress granules (SGs) are non-membranous assemblies of mRNAs and proteins expressed in response to various stressors to promote cell survival. In this study, SG formation was examined to confirm the effects of polyhexamethylene guanidine phosphate (PHMG), chloromethylisothiazolinone (CMIT), and particulate matter (PM10) in airway epithelial cells, A549, HPAEpiC, and BEAS-2B cells. SGs were not observed after CMIT, PHMG, and PM10 treatments, as determined by immunofluorescence microscopy. Moreover, there was no change in the phosphorylation of the translation initiation factor eIF2αfollowing treatment with PHMG, CMIT, and PM10. Taken together, our findings might help determine the biological hazards of these materials.

Keywords: Chloromethylisothiazolinone; Particulate matter; Polyhexamethylene guanidine phosphate; Stress granule.

Grants and funding

This research was supported by the Korea Environment Industry & Technology Institute (KEITI) through the Environment Health Action Program, funded by Korea Ministry of Environment (MOE) [grant number 2018001360003].