Snake extract-laden hemostatic bioadhesive gel cross-linked by visible light

Yicheng Guo; Ying Wang; Xiaohong Zhao; Xue Li; Quan Wang; Wen Zhong; Kibret Mequanint; Rixing Zhan; Malcolm Xing; Gaoxing Luo

doi:10.1126/sciadv.abf9635

Snake extract-laden hemostatic bioadhesive gel cross-linked by visible light

Sci Adv. 2021 Jul 14;7(29):eabf9635. doi: 10.1126/sciadv.abf9635. Print 2021 Jul.

Authors

Yicheng Guo^{1

2}, Ying Wang¹, Xiaohong Zhao¹, Xue Li¹, Quan Wang³, Wen Zhong⁴, Kibret Mequanint⁵, Rixing Zhan⁶, Malcolm Xing⁷, Gaoxing Luo⁶

Affiliations

¹ Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University (Army Medical University), Chongqing 400038, China.
² Department of Mechanical Engineering, University of Manitoba, Winnipeg R3T 2N2, Canada.
³ Department of Civil Engineering, Shantou University, Shantou 515063, China.
⁴ Department of Biosystems Engineering, University of Manitoba, Winnipeg R3T 2N2, Canada.
⁵ Department of Chemical and Biochemical Engineering and School of Biomedical Engineering, The University of Western Ontario, London N6A 5B9, Canada.
⁶ Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University (Army Medical University), Chongqing 400038, China. zhanrixing@sina.com logxw@yahoo.com malcolm.xing@umanitoba.ca.
⁷ Department of Mechanical Engineering, University of Manitoba, Winnipeg R3T 2N2, Canada. zhanrixing@sina.com logxw@yahoo.com malcolm.xing@umanitoba.ca.

Abstract

Bioadhesives reduce operation time and surgical complications. However, in the presence of blood, adhesion strength is often compromised. Inspired by the blood clotting activity of snake venom, we report a visible light-induced blood-resistant hemostatic adhesive (HAD) containing gelatin methacryloyl and reptilase, which is a hemocoagulase (HC) extracted from Bothrops atrox HAD leads to the activation and aggregation of platelets and efficiently transforms fibrinogen into fibrin to achieve rapid hemostasis and seal the tissue. Blood clotting time with HAD was about 45 s compared with 5 to 6 min without HAD. HAD instantaneously achieved hemostasis on liver incision (~45 s) and cut rat tail (~34 s) and reduced blood loss by 79 and 78%, respectively. HAD is also efficient in sealing severely injured liver and abdominal aorta. HAD has great potential to bridge injured tissues by combing hemostasis with adhesives.

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