Detection of chromosome instability by interphase FISH in mouse and human tissues

Raul Torres-Ruiz; Tatiana P Grazioso; Marta Brandt; Marta Martinez-Lage; Sandra Rodriguez-Perales; Nabil Djouder

doi:10.1016/j.xpro.2021.100631

Detection of chromosome instability by interphase FISH in mouse and human tissues

STAR Protoc. 2021 Jun 28;2(3):100631. doi: 10.1016/j.xpro.2021.100631. eCollection 2021 Sep 17.

Authors

Raul Torres-Ruiz¹, Tatiana P Grazioso², Marta Brandt², Marta Martinez-Lage¹, Sandra Rodriguez-Perales¹, Nabil Djouder²

Affiliations

¹ Molecular Cytogenetics and Genome Editing Unit, Human Cancer Genetics Program, Centro Nacional de Investigaciones Oncológicas (CNIO), 28029, Madrid, Spain.
² Molecular Oncology Programme, Growth Factors, Nutrients and Cancer Group, Centro Nacional Investigaciones Oncológicas (CNIO), Madrid, 28029, Spain.

Abstract

Chromosomal instability (CIN), a type of genomic instability, favors changes in chromosome number and structure and it is associated with the progression and initiation of multiple diseases, including cancer. Therefore, CIN identification and analysis represents a useful tool for cancer diagnosis and treatment. Here, we report an optimized molecular cytogenetic protocol to detect CIN in formalin-fixed, paraffin-embedded mouse and human tissues, using fluorescent in situ hybridization to visualize and quantify chromosomal alterations such as amplifications, deletions, and translocations. For complete information on the generation and use of this protocol, please refer to Brandt et al. (2018).

Keywords: Cell Biology; In Situ Hybridization; Microscopy; Molecular Biology; Single Cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromosomal Instability*
Chromosomes, Artificial, Bacterial
DNA / genetics
Electrophoresis, Agar Gel
In Situ Hybridization, Fluorescence / methods*
Interphase*
Mice
Paraffin Embedding

Substances

DNA