LC-MS based metabolomic approach for the efficient identification and relative quantification of bioavailable cocoa phenolics in human urine

Abstract

This study was designed to investigate the rate and extent of urinary excretion of cocoa phenolic metabolites after human intake using metabolomics approach. In this context, a feeding trial was conducted where urine samples were collected at different time points over 48-h period. Several biomarkers were highlighted in LC-MS based chemometrics using principal component (PCA) and partial least squares discriminant analysis (PLS-DA), which revealed the presence of both epicatechin and gut microbial phenyl-γ-valerolactones (PVLs) conjugated analogues. The presences of these metabolites segregated and grouped the samples based on cocoa and non-cocoa ingestion. Furthermore, semi quantification of major bioavailable metabolites was performed to determine the interindividual differences and assess the relative bioavailability of cocoa compounds in the human body. Our approach presented here is unique in displaying a combination of LC-MS based chemometrics visualization strategies, which revealed and identified significant biomarkers that could reduce the problems associated with data screening complexity.

Keywords: Cocoa human trial; LC-ESI-MS; Metabolomics; PCA; PLSDA; Phenolic Metabolites.