Chemistry-Assisted Proteomic Profiling of O-GlcNAcylation

Qiang Zhu; Wen Yi

doi:10.3389/fchem.2021.702260

Chemistry-Assisted Proteomic Profiling of O-GlcNAcylation

Front Chem. 2021 Jun 25:9:702260. doi: 10.3389/fchem.2021.702260. eCollection 2021.

Authors

Qiang Zhu¹, Wen Yi¹

Affiliation

¹ Department of Hepatobiliary and Pancreatic Surgery, Zhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Abstract

The modification on proteins with O-linked N-acetyl-β-D-glucosamine (O-GlcNAcylation) is essential for normal cell physiology. Dysregulation of O-GlcNAcylation leads to many human diseases, such as cancer, diabetes and neurodegenerative diseases. Recently, the functional role of O-GlcNAcylation in different physiological states has been elucidated due to the booming detection technologies. Chemical approaches for the enrichment of O-GlcNAcylated proteins combined with mass spectrometry-based proteomics enable the profiling of protein O-GlcNAcylation in a system-wide level. In this review, we summarize recent progresses on the enrichment and proteomic profiling of protein O-GlcNAcylation.

Keywords: O-GlcNAcylation; chemical tools; enrichment strategies; mass spectrometry; quantitative proteomics.

Publication types

Review