Three new polyketides from vasR2 gene over-expressed mutant strain of Verrucosispora sp. NS0172

Li-Ping Dai; Wen Li; Hao-Xin Wang; Chun-Hua Lu

doi:10.1016/S1875-5364(21)60053-5

Three new polyketides from vasR2 gene over-expressed mutant strain of Verrucosispora sp. NS0172

Chin J Nat Med. 2021 Jul;19(7):536-539. doi: 10.1016/S1875-5364(21)60053-5.

Authors

Li-Ping Dai¹, Wen Li², Hao-Xin Wang³, Chun-Hua Lu⁴

Affiliations

¹ Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China.
² State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
³ State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China. Electronic address: wanghaoxin@sdu.edu.cn.
⁴ Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China. Electronic address: ahua0966@sdu.edu.cn.

PMID: 34247777
DOI: 10.1016/S1875-5364(21)60053-5

Abstract

Over-expression of the pathway specific positive regulator gene is an effective way to activate silent gene cluster. In the curret study, the SARP family regulatory gene, vasR2, was over-expressed in strain Verrucosispora sp. NS0172 and the cryptic gene cluster responsible for the biosynthesis of pentaketide ansamycin was partially activated. Two tetraketides (1 and 2) and a triketide (3) ansamycins, together with five known compounds (4-8), were isolated and elucidated from strain NS0172OEvasR2. Their NMR data were completely assigned by analysis of their HR-ESI-MS and ¹H, ¹³C NMR, HMQC, HMBC and ¹H-¹H COSY spectra.

Keywords: Ansamycins; SARP family regulatory gene; Verrucosispora sp. NS0172.

MeSH terms

Micromonosporaceae* / genetics
Micromonosporaceae* / metabolism
Multigene Family
Polyketides* / metabolism
Rifabutin / metabolism*

Substances

Polyketides
Rifabutin