Lignans with NO inhibitory activity from Tinospora sinensis

Jun-Sheng Zhang; De-Feng Xu; Xin-Xin Cao; Yin-Yin Wang; Hua Zhang

doi:10.1016/S1875-5364(21)60049-3

Lignans with NO inhibitory activity from Tinospora sinensis

Chin J Nat Med. 2021 Jul;19(7):500-504. doi: 10.1016/S1875-5364(21)60049-3.

Authors

Jun-Sheng Zhang¹, De-Feng Xu², Xin-Xin Cao², Yin-Yin Wang², Hua Zhang³

Affiliations

¹ School of Biological Science and Technology, University of Jinan, Jinan 250022, China. Electronic address: 021872104120@ujn.edu.cn.
² School of Biological Science and Technology, University of Jinan, Jinan 250022, China.
³ School of Biological Science and Technology, University of Jinan, Jinan 250022, China. Electronic address: bio_zhangh@ujn.edu.cn.

PMID: 34247773
DOI: 10.1016/S1875-5364(21)60049-3

Abstract

Two new lignan glucosides, tinsinlignans A and B (1 and 2), two new oxyneolignans, tinsinlignans C and D (3 and 4), along with one known analogue (5), were isolated from the stems of Tinospora sinensis. The structures of the new compounds were elucidated based on analysis of spectroscopic data, and the absolute configuration of 1 was determined through electronic circular dichroism (ECD) calculation based on the time-dependent density functional theory (TD-DFT). Compounds 1-4 were evaluated for their inhibitory effects on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in murine RAW264.7 macrophage cells and compounds 1 and 2 exhibited moderate inhibitory activities with IC₅₀ values of 18.5 ± 2.0 and 28.8 ± 1.2 μmol·L^-1, respectively.

Keywords: ECD calculation; Lignan glucoside; NO inhibition; Oxyneolignan; Tinospora sinensis.

MeSH terms

Animals
Glucosides* / pharmacology
Lignans* / pharmacology
Lipopolysaccharides
Mice
Molecular Structure
Nitric Oxide
Phytochemicals / pharmacology
RAW 264.7 Cells
Tinospora* / chemistry

Substances

Glucosides
Lignans
Lipopolysaccharides
Phytochemicals
Nitric Oxide