Complete genome sequencing and comparative genome analysis of the extremely halophilic archaea, Haloterrigena daqingensis

Biotechnol Appl Biochem. 2022 Aug;69(4):1482-1488. doi: 10.1002/bab.2220. Epub 2021 Aug 1.

Abstract

In the present study, we report the complete genome sequencing of Haloterrigena daqingensis species. The genome of H. daqingensis JX313T consisted of a circular chromosome with three plasmids. The genome size and G+C content were estimated to be 3835796 bp and 61.7%, respectively. A total of 4158 genes were predicted with six rRNAs and 45 tRNAs. Metabolic pathway analysis suggests that H. daqingensis JX313T codes for all the necessary genes responsible to sustain its life at saline environment. The pan-genome analysis suggests that the number of singleton-gene between H. daqingensis and other Haloterrigena species varied. The study not only helps us understand H. daqingensis strategy for dealing with high stress, but it also provides an overview of its genomic makeup.

Keywords: Haloterrigena daqingensis; genome sequencing; halophilic archaea.

MeSH terms

  • DNA, Archaeal / genetics
  • Halobacteriaceae* / genetics
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Whole Genome Sequencing

Substances

  • DNA, Archaeal
  • RNA, Ribosomal, 16S

Supplementary concepts

  • Haloterrigena