Lower Somatic Mutation Levels in the λ Light-Chain Repertoires with Chronic HBV Infection

Binbin Hong; Qiaoling Liu; Qiulan Li; Lili Su; Lizhi Wang

doi:10.1155/2021/5525369

Lower Somatic Mutation Levels in the λ Light-Chain Repertoires with Chronic HBV Infection

Evid Based Complement Alternat Med. 2021 Jun 7:2021:5525369. doi: 10.1155/2021/5525369. eCollection 2021.

Authors

Binbin Hong¹, Qiaoling Liu¹, Qiulan Li¹, Lili Su¹, Lizhi Wang²

Affiliations

¹ Central Laboratory, Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China.
² Traditional Chinese Medicine Department, Rehabilitation Hospital, Nan'an, Quanzhou 362300, China.

Abstract

To investigate the characteristics of the immunoglobulin light-chain repertoires with chronic HBV infection, the high-throughput sequencing and IMGT/HighV-QUEST were adapted to analyze the κ (IgK) and λ (IgL) light-chain repertoires from the inactive HBV carriers (IHB) and the healthy adults (HH). The comparative analysis revealed high similarity between the κ light-chain repertoires of the HBV carriers and the healthy adults. Nevertheless, the proportion of IGLV genes with ≥90% identity as the germline genes was higher in the IgL light-chain repertoire of the IHB library compared with that of HH library (74.6% vs. 69.1%). Besides, the frequency of amino acid mutations in the CDR1 regions was significantly lower in the IgL light-chain repertoire of the IHB library than that of the HH library (65.52% vs. 56.0%). These results suggested the lower somatic mutation level in the IgL repertoire of IHB library, which might indicate the biased selection of IGLV genes in the IgL repertoire with chronic HBV infection. These findings might lead to a better understanding of the characteristics of the light-chain repertoires of HBV chronically infected individuals.