Immunohistochemical Analysis of Expression, Phosphorylation, and Nuclear Translocation of NF-κB Proteins in Human Tissues

Davide Vecchiotti; Daniela Verzella; Daria Capece; Jessica Cornice; Mauro Di Vito Nolfi; Barbara Di Francesco; Guido Franzoso; Edoardo Alesse; Francesca Zazzeroni

doi:10.1007/978-1-0716-1669-7_3

Immunohistochemical Analysis of Expression, Phosphorylation, and Nuclear Translocation of NF-κB Proteins in Human Tissues

Methods Mol Biol. 2021:2366:27-42. doi: 10.1007/978-1-0716-1669-7_3.

Affiliations

¹ Department of Biotechnological and Applied Clinical Sciences (DISCAB), University of L'Aquila, L'Aquila, Italy.
² Centre for Molecular Immunology and Inflammation, Department of Immunology and Inflammation, Imperial College London, London, UK.
³ Department of Biotechnological and Applied Clinical Sciences (DISCAB), University of L'Aquila, L'Aquila, Italy. francesca.zazzeroni@univaq.it.

^# Contributed equally.

PMID: 34236631
DOI: 10.1007/978-1-0716-1669-7_3

Abstract

Immunohistochemistry (IHC) is a technique aimed at detecting specific antigens on tissue sections by the use of targeting reagents labeled with reporter molecules. This technique allows a snapshot of the structure of tissue and determines the cellular and subcellular localization of a target antigen. This chapter describes how to identify and localize NF-κB proteins in human tissue using immunohistochemical staining on formalin-fixed paraffin-embedded and frozen tissue.

Keywords: Antibodies; Antigen retrieval; Detection; Fixation; Identification (localization); Microscope; NF-κB.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens
Formaldehyde
Humans
Immunohistochemistry
NF-kappa B / metabolism*
Paraffin Embedding
Phosphorylation
Tissue Fixation

Substances

Antigens
NF-kappa B
Formaldehyde