Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionation

Davide Vecchiotti; Daniela Verzella; Daria Capece; Mauro Di Vito Nolfi; Barbara Di Francesco; Jessica Cornice; Guido Franzoso; Edoardo Alesse; Francesca Zazzeroni

doi:10.1007/978-1-0716-1669-7_2

Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionation

Methods Mol Biol. 2021:2366:19-25. doi: 10.1007/978-1-0716-1669-7_2.

Authors

Davide Vecchiotti¹, Daniela Verzella¹, Daria Capece¹, Mauro Di Vito Nolfi¹, Barbara Di Francesco¹, Jessica Cornice¹, Guido Franzoso², Edoardo Alesse¹, Francesca Zazzeroni³

Affiliations

¹ Department of Biotechnological and Applied Clinical Sciences (DISCAB), University of L'Aquila, L'Aquila, Italy.
² Centre for Molecular Immunology and Inflammation, Department of Immunology and Inflammation, Imperial College London, London, UK.
³ Department of Biotechnological and Applied Clinical Sciences (DISCAB), University of L'Aquila, L'Aquila, Italy. francesca.zazzeroni@univaq.it.

PMID: 34236630
DOI: 10.1007/978-1-0716-1669-7_2

Abstract

Cell fractionation is a method used to study different cellular events like protein translocation and sequestration by disrupting cells and fractionating their contents, thus allowing an enrichment of the protein of interest. Using different concentrations of sucrose or detergent buffer formulations in combination with centrifugations, the cell fractions are separated based on their density and size.

Keywords: Centrifugation; Cytoplasmatic protein; NF-κB; Nuclear protein; Sucrose.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Fractionation*
NF-kappa B
Subcellular Fractions

Substances

NF-kappa B