Abstract
We demonstrate that a recombinase ribozyme achieves multiple functions in the same reaction network: self-reproduction, iterative elongation and circularization of other RNAs, leading to synthesis of diverse products predicted by a kinetic model. This shows that key mechanisms can be integrated and controlled toward Darwinian evolution in RNA reaction networks.
MeSH terms
-
Azoarcus / enzymology
-
Biocatalysis
-
Genetic Phenomena
-
High-Throughput Nucleotide Sequencing
-
Inverted Repeat Sequences
-
Kinetics
-
RNA / chemistry
-
RNA / genetics*
-
RNA, Bacterial / chemistry
-
RNA, Bacterial / genetics*
-
RNA, Catalytic / chemistry
-
RNA, Catalytic / genetics*
-
Recombinases / chemistry
-
Recombinases / genetics
Substances
-
RNA, Bacterial
-
RNA, Catalytic
-
Recombinases
-
RNA