The Preparation, Antioxidant Activity Evaluation, and Iron-Deficient Anemic Improvement of Oat (Avena sativa L.) Peptides-Ferrous Chelate

He Yuanqing; Yang Pengyao; Ding Yangyang; Chen Min; Guo Rui; Duan Yuqing; Zhang Haihui; Ma Haile

doi:10.3389/fnut.2021.687133

The Preparation, Antioxidant Activity Evaluation, and Iron-Deficient Anemic Improvement of Oat (Avena sativa L.) Peptides-Ferrous Chelate

Front Nutr. 2021 Jun 21:8:687133. doi: 10.3389/fnut.2021.687133. eCollection 2021.

Authors

He Yuanqing^{1

2}, Yang Pengyao¹, Ding Yangyang³, Chen Min¹, Guo Rui¹, Duan Yuqing¹, Zhang Haihui¹, Ma Haile¹

Affiliations

¹ College of Food Science and Biological Engineering, Jiangsu University, Zhenjiang, China.
² The Laboratory Animal Research Center, Jiangsu University, Zhenjiang, China.
³ School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, China.

Abstract

Iron-chelating peptides have been widely considered as one of the best iron supplements to alleviate the iron deficiency. In this study, a novel oat peptides-ferrous (OP-Fe²⁺) chelate was prepared from antioxidant oat peptides obtained in the laboratory of the authors. The optimal preparation condition was obtained through the single-factor and response surface methodology, and the chelating rate could reach up to 62.6%. After chelation, the OP-Fe²⁺ chelate exhibited a significantly higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity than oat peptides. It was discovered that the hemoglobin concentration and the number of red blood cell levels in OP-Fe²⁺-treated iron-deficient anemic (IDA) rats were significantly higher than untreated IDA rats. The OP-Fe²⁺ chelate could also improve the hypertrophy of the spleen, serum iron (SI), total iron and binding capacity, and serum ferritin levels in the IDA rats. In addition, the OP-Fe²⁺ treatment significantly increased the antioxidant activities of super oxidase and glutathione in the liver homogenate of the IDA rats. Therefore, the OP-Fe²⁺ chelate is an effective type of iron supplement for IDA rats, which could be a promising source with anti-anemia and antioxidant activity.

Keywords: antioxidant activity; iron supplement; iron-deficient anemic; oat peptide; peptides-ferrous chelate.