Narrowband diffuse reflectance spectroscopy in the 900-1000 nm wavelength region to quantify water and lipid content of turbid media

Jesse H Lam; Kelsey J Tu; Sehwan Kim

doi:10.1364/BOE.425451

Narrowband diffuse reflectance spectroscopy in the 900-1000 nm wavelength region to quantify water and lipid content of turbid media

Biomed Opt Express. 2021 May 4;12(6):3091-3102. doi: 10.1364/BOE.425451. eCollection 2021 Jun 1.

Authors

Jesse H Lam^{1

2}, Kelsey J Tu³, Sehwan Kim^{2

3}

Affiliations

¹ University of California, Irvine, Beckman Laser Institute, Department of Biomedical Engineering, Irvine, CA 92612, USA.
² Dankook University, Beckman Laser Institute Korea, School of Medicine, Cheonan, Chungnam, Republic of Korea.
³ Dankook University, Department of Biomedical Engineering, Cheonan, Chungnam, Republic of Korea.

Abstract

We report a narrow wavelength band diffuse reflectance spectroscopy (nb-DRS) method to determine water and fat ratios of scattering media in the 900-1000 nm range. This method was shown to be linearly correlated with absolute water and fat concentrations as tested on a set of turbid emulsion phantoms with a range of water and lipid compositions. Robustness to scattering assumptions was demonstrated and compared against measured scattering by a frequency-domain photon migration system. nb-DRS was also tested on ex-vivo porcine samples and compared against direct tissue water extraction by analytical chemistry methods. We speculate nb-DRS has potential applications in portable devices such as clinical and digital health wearables.