Antibacterial, Antibiofilm, and Antioxidant Activity of Polysaccharides Obtained from Fresh Sarcotesta of Ginkgo biloba: Bioactive Polysaccharide that Can Be Exploited as a Novel Biocontrol Agent

Hong Jiang; Zuxiang Luan; Zhaobing Fan; Xinliang Wu; Ziheng Xu; Tiezhong Zhou; Hongjun Wang

doi:10.1155/2021/5518403

Antibacterial, Antibiofilm, and Antioxidant Activity of Polysaccharides Obtained from Fresh Sarcotesta of Ginkgo biloba: Bioactive Polysaccharide that Can Be Exploited as a Novel Biocontrol Agent

Evid Based Complement Alternat Med. 2021 Jun 15:2021:5518403. doi: 10.1155/2021/5518403. eCollection 2021.

Authors

Hong Jiang¹, Zuxiang Luan², Zhaobing Fan³, Xinliang Wu⁴, Ziheng Xu⁵, Tiezhong Zhou¹, Hongjun Wang¹

Affiliations

¹ Institute of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University, Jinzhou 121001, Liaoning, China.
² School of Teacher Education, Nanning Normal University, Nanning 530001, Guangxi, China.
³ College of Pharmacy, Heze University, Heze 274015, Shandong, China.
⁴ Department of Pharmacy, Tianjin Baodi Hospital, Baodi Clinical College, Tianjin Medical University, Tianjin 301800, China.
⁵ Institute for Poultry Science and Health, Guangxi University, Nanning 530004, Guangxi, China.

Abstract

Staphylococcus aureus (S. aureus) biofilm plays an important role in the persistence of chronic infection due to its resistance to antibiotics. Because of their functional diversity, active polysaccharide is increasingly being applied as a biocontrol agent to inhibit the formation of biofilm by pathogens. In this study, a new polysaccharide, GBSPII-1, isolated from the fresh sarcotesta of Ginkgo biloba L. (G. biloba) was characterized and its effect on antibiofilm formation of S. aureus was examined in vitro. High-Performance Liquid Chromatography (HPLC) analysis showed that GBSPII-1 is an acidic heteropolysaccharide composed of mannose, rhamnose, glucose, glucuronic acid, and galacturonic acid. GBSPII-1 demonstrated a molecular weight of 34 kDa and may affect the accumulation of polysaccharide intercellular adhesion (PIA) by inhibiting icaA, icaB, icaC, and icaD gene expression at subinhibitory concentrations. Under 10 g/L, GBSPII-1 showed an antioxidant effect on the inhibition rate of H₂O₂-induced erythrocyte hemolysis and the scavenging rate of DPPH radicals was 76.5 ± 0.5% and 89.2 ± 0.26%, respectively. The findings obtained in this study indicate that GBSPII-1 has antibacterial effect, is a possible source of natural antioxidants, and may be a potential biocontrol agent for the design of new therapeutic strategies for biofilm-related S. aureus infections.