Transcriptomic and Weighted Gene Co-expression Correlation Network Analysis Reveal Resveratrol Biosynthesis Mechanisms Caused by Bud Sport in Grape Berry

Feng Leng; Yunling Ye; Jialing Zhou; Huijuan Jia; Xiaoheng Zhu; Jiayu Shi; Ziyue Zhang; Nan Shen; Li Wang

doi:10.3389/fpls.2021.690095

Transcriptomic and Weighted Gene Co-expression Correlation Network Analysis Reveal Resveratrol Biosynthesis Mechanisms Caused by Bud Sport in Grape Berry

Front Plant Sci. 2021 Jun 18:12:690095. doi: 10.3389/fpls.2021.690095. eCollection 2021.

Authors

Feng Leng¹, Yunling Ye¹, Jialing Zhou¹, Huijuan Jia², Xiaoheng Zhu², Jiayu Shi¹, Ziyue Zhang¹, Nan Shen¹, Li Wang¹

Affiliations

¹ College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
² Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement of the Ministry of Agriculture/Department of Horticulture, Zhejiang University, Hangzhou, China.

Abstract

Resveratrol is a natural polyphenol compound produced in response to biotic and abiotic stresses in grape berries. However, changes in resveratrol caused by bud sport in grapes are scarcely reported. In this study, trans-resveratrol and cis-resveratrol were identified and quantified in the grape berries of 'Summer Black' and its bud sport 'Nantaihutezao' from the veraison to ripening stages using ultra performance liquid chromatography-high resolution tandem mass spectrometry (UPLC-HRMS). We found that bud sport accumulates the trans-resveratrol earlier and increases the contents of cis-resveratrol in the earlier stages but decreases its contents in the later stages. Simultaneously, we used RNA-Seq to identify 51 transcripts involved in the stilbene pathways. In particular, we further identified 124 and 19 transcripts that negatively correlated with the contents of trans-resveratrol and cis-resveratrol, respectively, and four transcripts encoding F3'5'H that positively correlated with the contents of trans-resveratrol by weighted gene co-expression network analysis (WGCNA). These transcripts may play important roles in relation to the synergistic regulation of metabolisms of resveratrol. The results of this study can provide a theoretical basis for the genetic improvement of grapes.

Keywords: grape; resveratrol; transcriptome; ultra performance liquid chromatography-high resolution tandem mass spectrometry; weighted gene co-expression network analysis.